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Beilstein J. Org. Chem. 2015, 11, 1553–1560, doi:10.3762/bjoc.11.171
Graphical Abstract
Scheme 1: Synthesis of soluble support. Reagents and conditions: (i) CuI, sodium ascorbate, DMAC, 50 °C, 12 h...
Scheme 2: Activation of nucleosides. Reagents and conditions: (i) pyridine, dry dioxane, room temperature, 3 ...
Scheme 3: Synthesis of oligonucleotides. Reagents and conditions: (i) 4–6, 1-methylimidazole, dioxane, under N...
Figure 1: (A) HPLC traces for the precipitated support bearing phosphate-protected 3’-T-5’ monomer having the...
Figure 2: (A) HPLC traces for the precipitated support bearing phosphate-protected 3’-pTpdCBz-5’-O-DMTr (8a) ...
Figure 3: (A) HPLC traces for the precipitated support bearing phosphate-protected 3’-pTpdCBz-5’-OH dimer 8b,...
Scheme 4: Cleavage of oligonucleotides from support. Reagents and conditions: (i) tris(2-carboxyethyl)phosphi...
Beilstein J. Org. Chem. 2014, 10, 2279–2285, doi:10.3762/bjoc.10.237
Scheme 1: Synthesis of building blocks of the oligoribonucleotide synthesis. (i) TFA, aq THF, 0 °C; (ii) 2-me...
Scheme 2: (i) 6a, CuI, sodium ascorbate, DMAc; (ii) HCl in dioxane/MeOH 2:1, (iii) 5a', 4,5-dicyanoimidazole,...
Figure 1: HPLC traces referring to precipitation of the tetrapodal support bearing fully protected UU-dimers (...
Figure 2: HPLC traces referring to precipitation of the tetrapodal support bearing 5'-O-deprotected UU-dimers...
Figure 3: Negative ion ESIMS and HPLC traces of the isolated 3'-UUGCA-5'. For the MS over a wider mass range,...